Two-dimensional crystallization of avidin on biotinylated lipid monolayers
نویسندگان
چکیده
منابع مشابه
Purification and crystallization of avidin.
An improved purification, including crystallization, of avidin from hen's-egg white is described. The product bound 15.1mug of biotin/mg, corresponding to an equivalent weight of 16200. The amino acid composition showed an integral number of amino acid residues per 16200g, which suggested that each molecule of avidin (mol. wt. approx. 66000) contained four identical subunits.
متن کاملCrystallization on Self Assembled Monolayers
Over the past two decades, self assembled monolayers (SAMs) (Love et al., 2005; Smith et al., 2004; Ulman, 1996) have been extensively studied due to their many applications in various fields such as electrochemistry (Eckerman et al., 2010), biosensors (Nyquist et al., 2000), protein separation (Chun and Stroeve, 2002) and enantiomer separation (Mastai, 2009). SAMs are organic assemblies formed...
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The formation of mixed self-assembled monolayers (SAMs) from novel biotinylated alkylthiols (BAT) and diluent triethyleneglycol (TEG) alkylthiols on gold has been accomplished using aqueous solvents. We compare the effects of aqueous and ethanolic solvents on the binding performance of mixed SAMs and the integrity of monolayers formed. We use cyclic voltammetry (CV) and fluorescence microscopy ...
متن کاملAggregation of biotinylated polymeric microspheres induced by interaction with avidin
Monodisperse biotinylated poly(styrene-co-N-acryloxysuccinimide) microspheres were synthesized in aqueous solutions with a two-step method. Upon the addition of avidin solutions of different concentrations in phosphate buffer into the dispersed biotinylated microspheres, the microspheres aggregated rapidly due to the high binding affinity between biotin and avidin. The hydrodynamic diameter of ...
متن کاملTagging of avidin immobilized beads with biotinylated YAG:Ce3+ nanocrystal phosphor.
YAG:Ce3+ nanoparticles 9.5+/-1.2 nm in diameter have been synthesized from aluminium isopropoxide and acetates of yttrium and cerium in 1,4-butanediol (1,4-BD) by autoclave treatment at 300 degrees C for 2 h. After replacing 1,4-BD by ultrapure water, NH2 groups were introduced on the surface of YAG:Ce3+ nanoparticles by addition of 3-aminopropyltrimethoxysilane then biotinylation with sulfo-NH...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1995
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(95)80431-5